Fig. 5. Ratio of HL-60 cells phospholipids number of unsaturated and saturated bonds prior and post cryopreservation in DMSO +/ - Nig/Sal. HL-60 cells were pre-incubated for 24 hours in Nig, Sal or a combination of Nig/Sal prior freezing and cryopreserved in DMSO, DMSO + Nig, DMSO + Sal or DMSO + Nig/Sal. The fold change or ratio of the phospholipids is calculated by dividing the lipids mass of the post prior-cryopreservation in DMSO alone or +/- Nig/Sal by the prior-cryopreservation. (n=5; Mean +/- SEM).